Methylation analysis in patients with chronic myeloid leukemia in different medical institutions in Medellín
Keywords:
Leucemia mielogena crónica, metilación, progresión de la enfermedad, resistencia a medicamentosAbstract
Background: chronic myeloid leukemia (CML) is a chronic myeloproliferative neoplasm characterized by the presence of the Philadelphia chromosome. A specific treatment is designed as tyrosine kinase inhibitors (ITK's), which induces patients to have a hematologic, cytogenetic and molecular response. This disease is characterized by three clinical phases that result from the accumulation of genetic damage, both represented by point mutations and alterations in the karyotype; and epigenetic changes in genes such as ABL, OSCP1, PDLIM4, Npm2, ER and p15.
Objective: to describe the schemes of six gens in patients with chronic myeloid leukemia in different stages of the disease and treated with some ITK in two hospitals in Medellín.
Methods: a descriptive transversal study was conducted, in which 34 samples were collected at the convenience of patients with chronic myeloid leukemia (CML). To make the analysis of these samples methylation specific PCR was done.
Results: statistical differences in blood count data from both phases of the disease was found, a high frequency of methylated genes in accelerated phase was also found. p15 methylation, ABL, ER are independent of the stage of the disease. In patients treated with hydroxyurea, methylation of 100 % for OSCP1 and PDLIM4 genes was observed and this behavior was not observed in individuals treated with ITK'S. In patients who developed resistance to ITK's however was observed a higher percentage of methylation in genes OSCP1 and PDLIM4.
Conclusions: methylation in PDLIM4 and OSCP1 genes could be associated with poor prognosis possibly being associated with progression of chronic to accelerated phase and the development of resistance to ITK's.
DeCS: LEUKEMIA, MYELOGENOUS, CHRONIC, BCR-ABL POSITIVE; METHYLATION; PHILADELPHIA CHROMOSOME; HYDROXYUREA/therapeutic use; CROSS-SECTIONAL STUDIES.
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14 julio 2025