Molecular identification of human papilloma virus genotypes in patients with cervical cancer

Authors

  • Juan Carlos Piña Napal Universidad de Ciencias Médicas de Camaguey
  • Gustavo Crespo Campos Hospital Ginecoobstétrico de Camagüey, Universidad de Ciencias Médicas de Camagüey
  • Rafael Fando Calzado Centro Nacional de Investigaciones Científicas, La Habana
  • Gabriel Casanova Corona Universidad de Ciencias Médicas de Camagüey
  • Mileidy Curbelo Toledo Universidad de Ciencias Médicas de Camagüey
  • María Mercedes Guerra Rodríguez Universidad de Ciencias Médicas de Camagüey

Abstract

Background: it is demonstrated that the main cause of cervical cancer is high risk humanp virus. There is no precedent of molecular studies for the typing of Human Papilloma Virus in the population of Camagüey. Polymerase chain reaction is Molecular Biology technique that has been used traditionally for the clinical diagnosis and other purposes. This technique allows confirming the presence of papillomavirus´DNA in the total extracted DNA, from samples of patients with cervical cancer.
Objective: to demonstrate for the first time existing high-risk human papilloma virus genotypes that cause cervical cancer in female population of Camagüey, Cuba.
Methods: a prospective analytic study was conducted, in which 22 female patients of the province of Camagüey were studied. They received medical attention at Ana Betancourt Hospital. Identification and typing of the Human Papilloma Virus genotypes was carried through the molecular procedure Restriction Fragment Length Polymorphism.
Results: patients who presented exophytic lesions accounted for 63, 6%, 4, 5 % had endophytic type, and 31, 8 % presented other types. This study confirmed that high-risk Human Papilloma Virus genotypes existing in the province of Camagüey are genotypes 16 and 31, and the most frequent is 16.
Conclusions: this research is the first report of a molecular study of Human Papilloma Virus from samples of patients with cervical cancer in the province of Camagüey, Cuba. These results, along with the ones obtained by other authors, make an important contribution in the design of the increasingly effective therapeutic and preventive vaccine to an anticipated solution to cervical cancer in Cuba.
DeCS: POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; HUMAN PAPILLOMAVIRUS 16; UTERINE CERVICAL NEOPLASMS; ANALYTIC SAMPLE PREPARATION METHODS.

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Published

2016-06-23

How to Cite

1.
Piña Napal JC, Crespo Campos G, Fando Calzado R, Casanova Corona G, Curbelo Toledo M, Guerra Rodríguez MM. Molecular identification of human papilloma virus genotypes in patients with cervical cancer. Arch méd Camagüey [Internet]. 2016 Jun. 23 [cited 2025 Aug. 24];20(3):288-9. Available from: https://revistaamc.sld.cu/index.php/amc/article/view/4324

Issue

Vol. 20 No. 3 (2016)

Section

Original Articles

License

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