Morphometric indicators of the frontal cerebral cortex of Wistar rats' kits with pregestational diabetes

Authors

  • Leticia Mármol-Caballero Universidad de Ciencias Médicas. Facultad de Ciencias Médicas Mariana Grajales Coello. Departamento de Ciencias Básicas Biomédicas. Holguín, Cuba. https://orcid.org/0000-0002-6397-4211
  • Pedro Augusto Díaz-Rojas Universidad de Ciencias Médicas. Facultad de Ciencias Médicas Mariana Grajales Coello. Departamento de Ciencias Básicas Biomédicas. Holguín, Cuba. https://orcid.org/0000-0003-4897-363X
  • Doralny Peña-Marrero Universidad de Ciencias Médicas. Facultad de Ciencias Médicas Mariana Grajales Coello. Departamento de Ciencias Básicas Biomédicas. Holguín, Cuba. https://orcid.org/0000-0002-5192-7629
  • Liúdisis Silva-Jardínez Universidad de Ciencias Médicas. Facultad de Ciencias Médicas Mariana Grajales Coello. Departamento de Ciencias Básicas Biomédicas. Holguín, Cuba. https://orcid.org/0000-0001-9438-9894
  • Dunia Yailín Macareño-Ávila Universidad de Ciencias Médicas. Facultad de Ciencias Médicas Mariana Grajales Coello. Departamento de Ciencias Básicas Biomédicas. Holguín, Cuba. https://orcid.org/0000-0001-9203-1370

Abstract

Introduction: Diabetes is a disease that affects pregnancy causing fetal complications; within them congenital malformations are frequent. Due to the practical and ethical impossibility of studying this process in pregnant women, it is essential to carry out experimental studies using morphometric procedures to determine if diabetes affects neurodevelopment.
Objective: To characterize morphometrically the gray matter of kits from normal Wistar rats and those with pregestational diabetes mellitus.
Methods: A basic experimental study of a series of cases was carried out on 20 young Wistar rats, of which 10 were descendants of pregestational diabetes. Morphometric indicators of the nervous tissue were characterized as thickness of the cortex and nuclear indicators such as perimeter.
Results: The average height of the cortical gray matter showed a value of 1.224 ± 303.7 μm for the control group and 1.014 ± 376.0 μm for the cases. When applying the mean difference test, a significant difference was found (p ≤ 0.05) in favor of the control group. The values of the measurement of the nuclear perimeter in the control group was 42.80 ± 7.23 μm and in the experimental group the average was 39.68 ± 6.52 μm. When applying the mean different test, a significant difference was found (p ≤ 0.05) at favor of control group presenting greater nuclear perimeter.
Conclusions: The greatest cortical thickness and nuclear perimeter corresponded to the control group, evidencing the deleterious effect os diabetes mellitus on neurodevelopment.

DeCS: DIABETES MELLITUS; RATS, WISTAR; GRAY MATTER; ANIMALS, LABORATORY; PREGNANCY.

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Published

2022-12-23

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1.
Mármol-Caballero L, Díaz-Rojas PA, Peña-Marrero D, Silva-Jardínez L, Macareño-Ávila DY. Morphometric indicators of the frontal cerebral cortex of Wistar rats’ kits with pregestational diabetes. Arch méd Camagüey [Internet]. 2022 Dec. 23 [cited 2026 Feb. 12];27:e9506. Available from: https://revistaamc.sld.cu/index.php/amc/article/view/9506

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