Gram and urease for the diagnosis of helicobacter pylori in gastroduodenal samples

Authors

  • Marta Julia López Reginfo Hospital Clínico Quirúrgico Docente "Saturnino Lora"
  • Leonor Aties López
  • Estrella Tatiana Sánchez Ojeda
  • María Caridad Mariño Castellanos
  • Yadmila Duret Gala

Keywords:

helicobacter pylori, Gram, urea, diagnóstico microbiológico.

Abstract

Background: gastroduodenal affection and gastric cancer are diseases which are more frequent every day. Helicobacter pylori is the responsible microorganism for the largest number of cases.
Objective: to determine the presence of helicobacter pylori in grastroduodenal biopsy samples from the application of microbiological techniques: urease test and Gram staining.
Methods: in 2012 a cross-sectional descriptive study was conducted to determine the presence of helicobacter pylori in gastroduodenal biopsy samples through microbiological techniques in the microbiology laboratory of “Saturnino Lora” hospital in Santiago de Cuba.
Results: from the whole group of patients studied a predominance of 98 women that account for 66,6 % was found. From the bad habits assessed the one with the highest percentage was coffee consumption, with 132 cases which represent 89.8 %. The most notable symptom found was epigastralgia. After undergoing the referred tests of the study (urease test and Gram staining), 66,7 % of positive samples with urease test and 79,6 % with Gram staining were obtained.
Conclusions: morbidity prevailed in female sex in the 31 to 45 age group. Gram staining is more sensitive than urease test. Patients with chronic gastritis showed gram-positive results to helicobacter pylori.
DeCS: UREASE; HELICOBACTER PYLORI; MICROBIOLOGICAL TECHNIQUES; PEPTIC ULCER; EPIDEMIOLOGY, DESCRIPTIVE.

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Author Biography

Marta Julia López Reginfo, Hospital Clínico Quirúrgico Docente "Saturnino Lora"

Licenciada en Ciecias Médicas. Especialidad Microbiología. Máster en Enfermedades Infecciosas

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Published

2016-10-25

How to Cite

1.
López Reginfo MJ, Aties López L, Sánchez Ojeda ET, Mariño Castellanos MC, Duret Gala Y. Gram and urease for the diagnosis of helicobacter pylori in gastroduodenal samples. Arch méd Camagüey [Internet]. 2016 Oct. 25 [cited 2025 Aug. 21];20(5):488-95. Available from: https://revistaamc.sld.cu/index.php/amc/article/view/4658

Issue

Vol. 20 No. 5 (2016)

Section

Original Articles

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