Platelet functionality of autologous platelet-rich plasma with the use of epinephrine as an agonist
Abstract
Introduction: Light transmission aggregometry consists of adding an agonist to the patient's platelet-rich plasma and measuring the aggregation of platelets, which is detected thanks to an optical detection system.
Objective: To determine platelet functionality through aggregation with epinephrine as an agonist in platelet-rich plasma samples by measuring optical density.
Methods: A descriptive cross-sectional study was carried out. For platelet aggregation, epinephrine was used as an agonist. The optical density was measured on samples in apparently healthy subjects with a normal platelet range (150-450 X109/L) for three days and three temperatures. The aggregating substance was epinephrine, with a concentration of 2.5 x 10-5 M quantitatively.
Results: The average optical density in platelet aggregation induced by epinephrine as an agonist at three times and three temperatures showed significant differences (p=0.02-0.05). Where the agonist was not used, the significance was greater than 0.05.
Conclusions: The functionality of autologous platelet-rich plasma was evidenced with a platelet aggregation study; these results allowed us to have a complete and general vision of the functionality of the final product.
DeCS: PLATELET AGGREGATION; PLATELET-RICH PLASMA: EPINEPHRINE; RECEPTORS, ADRENERGIC; PLATELET FUNCTION TESTS.
Downloads
References
1. Born GV, Cross MJ. The aggregation of blood platelets. J Physiol [Internet]. 1963 [citado 20 May 2024];168: 178-195. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1359417/pdf/jphysiol01214-0203.pdf
2. Guevara Arismendi NM, Escobar Gallo GE, Campuzano Maya G. Clinical utility of platelet aggregometry. Med Labor [Internet]. 2012 [citado 20 May 2024];18(7-8):[about 21 p.]. Disponible en: https://www.medigraphic.com/pdfs/medlab/myl-2012/myl127-8b.pdf
3. Malarczyk D, Odnoczko E. Light transmission aggregometry in the diagnosis of thrombocytopathy. J Tran Med [Internet]. 2023 [citado 20 May 2024];16(3):[about 21 p.]. Disponible en: https://journals.viamedica.pl/journal_of_transfusion_medicine/article/view/JTM.2023.0006/72696
4. Sachs UJ, Röder L, Cooper N, Radon Ch, Kolde HJ. Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison. TH Open [Internet]. 2023 [citado 7 May 2024];7(1):[about 9 p.]. Disponible en: https://www.thiemeconnect.com/products/ejournals/pdf/10.1055/s-0043-1762588.pdf
5. Denessen EJS, Van Den Kerkhof DL, Jeurissen MLJ, Wetzels RJH, Verhezen P WM, Henskens YMC. Determining the optimal storage time and temperature for performing platelet function assays and global hemostasis assays. Platelets [Internet]. 2022 [citado 7 May 2024];33(3):416-424. Disponible en: https://www.tandfonline.com/doi/epdf/10.1080/09537104.2021.1934666?needAccess=true&role=button
6. Lussana F, Femia EA, Pugliano M, Podda G, Razzari C, Maugeri N; et al. Evaluation of platelet function in essential thrombocythemia under different analytical conditions. Platelets [Internet]. 2020 [citado 7 May 2024];31(2):[about 8 p.]. Disponible en: https://www.tandfonline.com/doi/abs/10.1080/09537104.2019.1584668
7. Stépanian A, Fischer F, Flaujac C, Eschwège V, Delassasseigne C, Leflem L; et al. Light transmission aggregometry for platelet function testing: position paper on current recommendations and French proposals for accreditation. Platelets [Internet]. 2024[citado 6 Marz 2024];35(1):[about 25 p.].Disponible en: https://pubmed.ncbi.nlm.nih.gov/39555668/
8. Zhou Y, Yasumoto A, Lei C, Huang CJ, Kobayash H, Wu Y; et al. Classification of platelet aggregates by agonist type. BioRxiv [Internet]. 2019 [citado 22 May 2024];2(8) :[about 7 p.]. Disponible en: https://www.biorxiv.org/content/10.1101/805408v2.full.pdf
9. Zhou L, Schmaier AH. Platelet aggregation testing in platelet-rich plasma: description of procedures with the aim to develop standards in the field. Am J Clin Pathol [Internet]. 2005[citado 23 May 2024];123(2): 172-183. Disponible en: https://pubmed.ncbi.nlm.nih.gov/15842039/
10. Lussana F, Femia EA, Pugliano M, Podda G, Razzari C, Maugeri N; et al. Evaluation of platelet function in essential thrombocythemia under different analytical conditions. Platelets [Internet]. 2020 [citado 8 May 2023];31(2):[about 4 p.]. Disponible en: https://www.tandfonline.com/doi/abs/10.1080/09537104.2019.1584668
11. Le Blanc J, Mullier F, Vayne C, Lordkipanidzé M. Advances in Platelet Function Testing—Light Transmission Aggregometry and Beyond. J Clin Med [Internet]. 2020 [citado 8 May2023];9(8):[about 2 p.]. Disponible en: https://www.mdpi.com/2077-0383/9/8/2636
12. Hayward C, Moffat K, Raby A, Israels S, Plumhoff E, Flynn G; et al. Development of North American Consensus Guidelines for medical laboratories that perform andinterpret platelet function testing using Light Transmission Aggregometry. American Journal of Clinical Pathology [Internet]. 2010 [citado 28 May 2024]; 134 :[about 7 p.]. Available from: https://academic.oup.com/ajcp/article/134/6/955/1760860?login=false
13. Linnemann B, Schwonberg J, Mani H, Prochnow S, Lindhoff-Last E. Standardization of light transmittance aggregometry for monitoring antiplatelettherapy: an adjusttment for platelet count is not necessary. J Thromb Haem [Internet]. 2008 [citado 8 May 2024];6(4):[about 2 p.]. Disponible en:: https://www.sciencedirect.com/science/article/pii/S1538783622105416
14. Checa Rojas A. Método: Conversión de las fuerzas g (RCF) a revoluciones por minuto (rpm) y Equilibrio del rotor [Internet]. Estados Unidos: Conogasi.org; 2018 [citado 6 May 2024]. Disponible: https://conogasi.org/articulos/metodo-conversion-de-las-fuerzas-g-rcf-a-revoluciones-por-minuto-rpm-y-equilibrio-del-rotor/.
15. Sánchez Acero FA. Prueba Anova para datos no Paramétricos de Friedman en JASP [tesis]. Colombia: Fundación Universitaria Konrand Lorenz; 2020 [citado 10 julio 2024]. Disponible en: https://repositorio.konradlorenz.edu.co/handle/001/2491
16. Asociación Médica Mundial. Declaración de Helsinki de la AMM. Principios éticos para las investigaciones médicas en seres humanos [Internet]. Brasil: Asamblea General Fortaleza; 2013 [citado 20 May 2024]. Disponible en: https://www.wma.net/es/policies-post/declaracion-de-helsinki-de-la-amm-principios-eticos-para-las-investigaciones-medicas-en-seres-humanos/
17. Mesa González L, Castañeda Travieso M, Montero López M, Mojena López X, Ricardo Ricardo N, Jiménez Sosa M. Estudio de agregación plaquetaria con diferentes agonistas. Valores de referencia. Rev cuban med [Internet]. 2017 [citado 10 May 2024];56(1):[aprox. 5 p.]. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034-75232017000100005
18. Gómez Gómez B, Rodríguez Weber FL, Díaz Greene EJ. Fisiología plaquetaria, agregometría plaquetaria y su utilidad clínica. Med Interna Méx [Internet]. 2018 [citado 10 May 2024];34(2):[aprox. 3 p.]. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0186-48662018000200007&lng=es
19. Silva LL da, D'Amico EA. Estudo comparativo entre agregação plaquetária por turbidimetria e impedância elétrica em pacientes sob terapia antiplaquetária ? base de ácido acetilsalicílico. Rev Bras Hematol Hemoter [Internet]. 2010 [citado 10 May 2024];32(6): [aprox. 6 p.]. Disponible en: https://www.scielo.br/j/rbhh/a/3bVg58kJvVMPG9LvRWrLjGF/?format=pdf&lang=en
20. Tsoupras A, Zabetakis I, Lordan R. Platelet aggregometry assay for evaluating the effects of platelet agonists and antiplatelet compounds on platelet function in vitro. MethodsX [Internet]. 2018 [citado 19 May 2024];26(6):[about 2 p.]. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6312786/.
21. García Chávez J, Hernández Juárez J, Sánchez Jara B, García Lee MT, Rodríguez Castillejos C, Montiel Cervantes L; et al. Consenso mexicano para el diagnóstico y tratamiento de la trombastenia de Glanzmann. Gac Méd Méx [Internet]. 2022 [citado 19 May 2024];158(spe4): [aprox. 5 p.]. Disponible en: https://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0016-38132022001000001
22. Platton S, McCormick Á, Bukht M, Gurney D, Holding I, Moore GW. A multicenter study to evaluate automated platelet aggregometry on Sysmex CS-series coagulation analyzers-preliminary findings. Res Pract Thromb Haemost [Internet]. 2018 [citado 10 May 2024];22:[about 3 p.]Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178605/pdf/RTH2-2-778.pdf
Published
How to Cite
Issue
Section
License
Copyright: Camagüey Medical Archive Magazine, offers immediately after being indexed in the SciELO Project; Open access to the full text of the articles under the principle of making available and free the research to promote the exchange of global knowledge and contribute to a greater extension, publication, evaluation and extensive use of the articles that can be used without purpose As long as reference is made to the primary source.
Conflicts of interest: authors must declare in a mandatory manner the presence or not of conflicts of interest in relation to the investigation presented.
(Download Statement of potential conflicts of interest)
The Revista Archivo Médico de Camagüey is under a License Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 International (CC BY 4.0).
This license allows others to distribute, to mix, to adjust and to build from its work, even for commercial purposes, as long as it is recognized the authorship of the original creation. This is the most helpful license offered. Recommended for maximum dissemination and use of licensed materials. The full license can be found at: https://creativecommons.org/licenses/
22 julio 2025